Počet záznamů: 1
TRAIL receptor upregulation and the implication of KRAS/BRAF mutations in human colon cancer tumours
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SYSNO ASEP 0334035 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název TRAIL receptor upregulation and the implication of KRAS/BRAF mutations in human colon cancer tumours Tvůrce(i) Oikonomou, E. (GR)
Kosmidou, V. (GR)
Katseli, A. (GR)
Kothonidis, K. (GR)
Mourtzoukou, D. (GR)
Kontogeorgos, G. (GR)
Anděra, Ladislav (UMG-J) RID
Zografos, G. (GR)
Pintzas, A. (GR)Celkový počet autorů 9 Zdroj.dok. International Journal of Cancer. - : Wiley - ISSN 0020-7136
Roč. 125, č. 9 (2009), s. 2127-2135Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova colorectal tumours ; TRAIL receptors expression ; KRAS/ BRAF oncogenic mutations Vědní obor RIV EB - Genetika a molekulární biologie CEP 1M0506 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000270750000016 DOI 10.1002/ijc.24613 Anotace TRAIL raises hopes as a promising anti-tumor agent due to its selectivity toward cancer cells. Since TRAIL receptor availability can be analogous to ligand efficacy, we performed RT-PCR and immunohistochemical analysis of DR4 and DR5 in 51 colon cancer biopsy specimens and respective normal mucosa. The results showed that DR4 and DR5 were significantly upregulated in 37 and 47% of the tumor samples respectively, while both DR4 and DR5 were co-instantaneously upregulated in 31% of the samples analyzed. Positive transcriptional regulation of DRs was recorded as early as Dukes' A stage. Possible contribution of frequent oncogenic mutations in the MAPK pathway was investigated by direct sequencing in all 51 tumors. Samples (6/8) hosting either a KRAS(G12V) or BRAF(V600E) mutation, significantly amplified the upregulated expression of DR4 and DR5, showing strong inter-relation between overexpression and presence of oncogenic KRAS/ BRAF mutations and DRs expression. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2010
Počet záznamů: 1