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Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage

SYSNO ASEP	0334003
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage
Tvůrce(i)	Toralová, Tereza (UZFG-Y)_ORCID Šušor, Andrej (UZFG-Y)_{RID, ORCID} Němcová, Lucie (UZFG-Y)_RID Kepková, Kateřina (UZFG-Y) Kaňka, Jiří (UZFG-Y)_{RID, ORCID}
Zdroj.dok.	Reproduction. - : BioScientifica - ISSN 1470-1626 Roč. 138, č. 5 (2009), s. 783-791
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	CENPF ; bovine embryo ; preimplantation development
Vědní obor RIV	GI - Šlechtění a plemenářství hospodářských zvířat
CEP	GA523/06/1226 GA ČR - Grantová agentura ČR
	GD204/05/H023 GA ČR - Grantová agentura ČR
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000271572000004
DOI	10.1530/REP-09-0234
Anotace	The paper deals with the role of CENPF/mitosin during preimplantation development of cattle. We focused on monitoring of developmental competence of bovine embryos after injection of CENPF dsRNA into the zygotes. An average decrease of CENPF mRNA abundance by 94.9% or more and an extensive decline in immunofluorescence staining intensity was detected relative to controls. There was no disparity between the groups in the developmental competence before the 8-cell stage, where embryonic genome activation (EGA) in bovines takes place. However, the developmental competence rapidly decreased then and only 28.1% of CENPF dsRNA injected 8-cell embryos were able to develop further (controls 72.0%). We have further shown that in bovine post-EGA embryos CENPF protein is expressed in the same manner as in somatic cells. However, the protein in pre-EGA embryos is likely not degraded at the end of cell cycle, which enables the development of embryos till 8-cell stage.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2010

Počet záznamů: 1