Počet záznamů: 1
p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 regulates its subcellular localization
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SYSNO ASEP 0333651 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název p300-mediated acetylation of the Rothmund-Thomson-syndrome gene product RECQL4 regulates its subcellular localization Tvůrce(i) Dietschy, T. (CA)
Shevelev, Igor (UMG-J)
Pena-Diaz, J. (CH)
Hühn, D. (CH)
Kuenzle, S. (CA)
Mak, R. (CA)
Miah, M.F. (CA)
Hess, D. (CH)
Fey, M. (CH)
Hottiger, M.O. (CH)
Janščák, Pavel (UMG-J) RID
Stagljar, I. (CA)Celkový počet autorů 12 Zdroj.dok. Journal of Cell Science. - : Company of Biologists - ISSN 0021-9533
Roč. 122, Pt 8 (2009), s. 1258-1267Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova RECQL4 ; RecQ helicases ; Genome stability ; p300 ; Protein acetylation Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000264777700023 DOI 10.1242/jcs.037747 Anotace RECQL4 belongs to the conserved RecQ family of DNA helicases playing important roles in the maintenance of genome stability. Although genetic alterations in RECQL4 gene are reported to be associated with three autosomal recessive disorders, the molecular role of RECQL4 is still poorly understood. We show that RECQL4 specifically interacts with histone acetyltransferase p300 (p300 HAT) in vivo and in vitro and that p300 acetylates one or more lysine residues of RECQL4. We also report that these residues lie within a short motif of 30 aa essential for the nuclear localization of RECQL4. Acetylation of RECQL4 by p300 in vivo leads to a significant shift of a proportion of RECQL4 protein from the nucleus to the cytoplasm. This is a result of RECQL4 inability to be imported into the nucleus. Our results are the first evidence of post-translational modification of RECQL4 and suggest that acetylation of RECQL4 by p300 regulates trafficking of RECQL4 between the nucleus and the cytoplasm. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2010
Počet záznamů: 1