Počet záznamů: 1
Complementation analysis of triple gene block of Potato virus S (PVS) revealed its capability to support systemic infection and aphid transmissibility of recombinant Potato virus X
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SYSNO ASEP 0330919 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Complementation analysis of triple gene block of Potato virus S (PVS) revealed its capability to support systemic infection and aphid transmissibility of recombinant Potato virus X Tvůrce(i) Matoušek, Jaroslav (BC-A) RID, ORCID
Schubert, J. (DE)
Dědič, P. (CZ)Celkový počet autorů 3 Zdroj.dok. Virus Research. - : Elsevier - ISSN 0168-1702
Roč. 146, č. 1 (2009), s. 81-88Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Recombinant virus ; Virus host range ; Potato virus X ; Potato virus S Vědní obor RIV EE - Mikrobiologie, virologie CEP QF3109 GA MZe - Ministerstvo zemědělství 1QS500510558 GA AV ČR - Akademie věd CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000272098700011 DOI 10.1016/j.virusres.2009.09.003 Anotace Specific differences were found in triple gene block (TGB) sequences derived from isolates of ordinary Potato virus S (PVS-O) and Chenopodium-systemic (PVS-CS). In order to investigate functional divergence of PVS TGB variants, they were propagated in chimeric Potato virus X (PVX). Both PVS TGB variants partly complemented PVX TGB, but multiplied to lower titer than the wild-type PVX in N. benthamiana showing attenuated symptoms. From the propagation of recombinant PVX variants in different host and non-host plant species we assume there are additional determinants outside the TGB. Transmission by the aphid Myzus persicae in the recombinant virus was obtained by replacing PVX TGB by TGB from the PVS-CS isolate. These results show the potential function of Carlavirus TGB in aphid transmissibility and underlines the possible biological risks from certain recombinant virus variants. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2010
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