Počet záznamů: 1
Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping
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SYSNO ASEP 0330525 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping Překlad názvu Rozsáhlá konzervovaná syntenie genů mezi karyotypy Manduca sexta a Bombyx mori prokázaná mapováním metodou BAC-FISH Tvůrce(i) Yasukochi, Y. (JP)
Tanaka-Okuyama, M. (JP)
Shibata, F. (JP)
Yoshido, A. (JP)
Marec, František (BC-A) RID, ORCID
Wu, Ch. (US)
Zhang, H. (US)
Goldsmith, M. R. (US)
Sahara, K. (JP)Celkový počet autorů 9 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 4, č. 10 (2009), e7465Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Manduca sexta Vědní obor RIV EB - Genetika a molekulární biologie CEP GA206/06/1860 GA ČR - Grantová agentura ČR IAA600960925 GA AV ČR - Akademie věd CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000270847800007 DOI 10.1371/journal.pone.0007465 Anotace We used fluorescence in situ hybridization (FISH) with bacterial artificial chromosome (BAC) probes for physical mapping of genes and comparative genome analysis in Lepidoptera. We isolated BAC clones containing orthologs of single-copy genes of the silkworm, Bombyx mori, from a BAC library of the tobacco hornworm, Manduca sexta. We then constructed a BAC-FISH karyotype identifying all 28 chromosomes of M. sexta by mapping 124 loci using the corresponding BAC clones. BACprobes from M. sexta also generated clear signals on the corresponding chromosomes of a related species, Agrius convolvuli. Comparison of the M. sexta BAC physical map with the linkage map of B. mori pointed to conserved synteny including conserved gene order in most chromosomes. Only three inversions, three translocations, and two fission/fusion events were estimated to have occurred after the divergence of Bombycidae and Sphingidae. These results add to accumulating evidence for the stability of lepidopteran genomes. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2010
Počet záznamů: 1