Počet záznamů: 1
Stepwise DNA Methylation Changes Are Linked to Escape from Defined Proliferation Barriers and Mammary Epithelial Cell Immortalization
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SYSNO ASEP 0329667 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Stepwise DNA Methylation Changes Are Linked to Escape from Defined Proliferation Barriers and Mammary Epithelial Cell Immortalization Tvůrce(i) Novák, Petr (BC-A) RID, ORCID
Jensen, T.J. (US)
Garbe, J. (US)
Sampfer, M.R. (US)
Futscher, B. W. (US)Celkový počet autorů 5 Zdroj.dok. Cancer Research. - : American Association for Cancer Research - ISSN 0008-5472
Roč. 69, č. 12 (2009), s. 5251-5258Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Epigenetics ; Breast Cancer ; DNA methylation Vědní obor RIV EB - Genetika a molekulární biologie CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000267506400042 DOI 10.1158/0008-5472.CAN-08-4977 Anotace We analyzed genome-wide DNA methylation patterns in an isogenic human mammary epithelial cell (HMEC) culture model of transformation. To acquire immortality and malignancy, the cultured finite lifespan HMEC must overcome two distinct proliferation barriers. Our analysis of HMEC transitioning from finite lifespan to malignantly transformed showed that aberrant DNA methylation changes occur in a stepwise fashion early in the transformation process. The first aberrant DNA methylation step coincides with overcoming stasis, and results in few to hundreds of changes, depending on how stasis was overcome. A second step coincides with immortalization and results in hundreds of additional DNA methylation changes regardless of the immortalization pathway. A majority of these DNA methylation changes are also found in malignant breast cancer cells. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2010
Počet záznamů: 1