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HIGH-THROUGHPUT SCREENING TECHNOLOGY FOR MONITORING PHYTOHORMONE PRODUCTION IN MICROALGAE

SYSNO ASEP	0328778
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	HIGH-THROUGHPUT SCREENING TECHNOLOGY FOR MONITORING PHYTOHORMONE PRODUCTION IN MICROALGAE
Překlad názvu	Vysoce účinná technologie monitoringu fytohormonů v mikrořasách
Tvůrce(i)	Jirásková, D. (CZ) Poulíčková, A. (CZ) Novák, Ondřej (UEB-Q)_{RID, ORCID, SAI} Sedláková, Kamila (UEB-Q) Hradecká, Veronika (UEB-Q) Strnad, Miroslav (UEB-Q)_{RID, ORCID}
Zdroj.dok.	Journal of Phycology. - : Wiley - ISSN 0022-3646 Roč. 45, č. 1 (2009), s. 108-118
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	abscisic acid ; auxins ; cytokinins
Vědní obor RIV	BO - Biofyzika
CEP	GA206/09/1284 GA ČR - Grantová agentura ČR
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000263333500011
DOI	10.1111/j.1529-8817.2008.00615.x
Anotace	New miniaturized techniques for multiplying microalgae and estimating their phytohormone production were developed; in these methods, the strains to be tested are cultivated in microtitre plates, and the phytohormones in suspensions of the cultures are measured by direct ELISAs. Specific and sensitive ELISAs for determining abscisic acid (ABA), indole-3-acetic acid (IAA), cis- and trans-zeatin riboside, isopentenyladenosine (iPR), and other less common cytokinins were developed for this purpose. Polyclonal antibodies used in the ABA and IAA assays were raised against C1- and C1‘- conjugates of the compounds with BSA, respectively, and thus were specific for the free acids and their respective C1-derivatives. The use of cytokinin ribosides coupled via their sugar residues to BSA as haptens generally led to antibodies that bound free bases, 9-glycosides and nucleotides, but with high specificity for the corresponding N6-side chains.
Pracoviště	Ústav experimentální botaniky
Kontakt	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Rok sběru	2010

Počet záznamů: 1