Počet záznamů: 1
Enrichment of rat oligodendrocyte progenitor cells by magnetic cell sorting
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SYSNO ASEP 0328388 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Enrichment of rat oligodendrocyte progenitor cells by magnetic cell sorting Tvůrce(i) Čížková, D. (SK)
Čížek, M. (SK)
Nagyová, M. (SK)
Slovinská, L. (SK)
Novotná, I. (SK)
Jergová, S. (SK)
Radoňák, J. (SK)
Hlučilová, Jana (UZFG-Y) RID, ORCID
Vanický, I. (SK)Zdroj.dok. Journal of Neuroscience Methods. - : Elsevier - ISSN 0165-0270
Roč. 184, č. 1 (2009), s. 88-94Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Oligodendrocytes progenitors Lineage ; Magnetic separation Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy CEP MEB0808108 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000271053400011 DOI 10.1016/j.neumeth.2009.07.030 Anotace In the present study we have used magnetic cell sorting (MACs) technology to generate enriched oligodendroglial cell populations from the embryonic (E16) rat spinal cord. Target cells were separated by positive selection, using specific A2B5 antibody-labeled MicroBeads achieving optimal recovery and high purity of pro-oligodendroglial cells. Based on immunocytochemical analyses for oligodendroglial developmental markers (A2B5, NG2, RIP and MBP) we were able to characterize and quantify oligodendroglial progenitors (OPCs) and mature oligodendroglial cells in: (i) unseparated heterogeneous population of NSCs, or in (ii) antigen–antibody separated NSCs. Our results showed that MACs technology enable us to gain enriched OPCs from heterogeneous population of spinal NSCs, resulting in a 58–61% of mature oligodendrocytes content (MBP+, RIP+) in comparison to 6–12% of oligodendroglial cells acquired from unseparated population. In addition, the enriched OPCs could be cultured in vitro for several >8 passages, giving rise to a high number of newly formed spheres, as well as high expansion potential. These experiments indicate that MACs technology provide a feasible approach for experimental cell enrichment of desired oligodendroglial progeny, which may be used in future trials for cell-based therapies to treat spinal cord injury. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2010
Počet záznamů: 1