Počet záznamů: 1
Magnesium interference and different efficiencies of diastereoisomeric cluster formation in phenylalanine enantiomeric discrimination by the kinetic method
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SYSNO ASEP 0328370 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Magnesium interference and different efficiencies of diastereoisomeric cluster formation in phenylalanine enantiomeric discrimination by the kinetic method Překlad názvu Interference hořčíku při stanovení diastereomerní čistoty fenylalaninu , kinetickou metodou Tvůrce(i) Ranc, V. (CZ)
Havlíček, Vladimír (MBU-M) RID, ORCID
Bednář, P. (CZ)
Lemr, Karel (MBU-M) RID, ORCIDZdroj.dok. International Journal of Mass Spectrometry. - : Elsevier - ISSN 1387-3806
Roč. 280, 1-3 (2009), s. 213-217Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova enantiomeric dicrimination ; kinetic method ; mass spectrometry Vědní obor RIV CB - Analytická chemie, separace CEP LC07017 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA203/07/0765 GA ČR - Grantová agentura ČR CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000263195500034 DOI 10.1016/j.ijms.2008.09.009 Anotace The kinetic method has been applied for determination of d-Phe/l-Phe enantiomeric ratio. Discrimination of enantiomers was inferred from product ion mass spectra of trimeric cluster ions containing the analyte (l,d-Phe), Cu2 as a central metal and l-Trp as a chiral reference ligand. Unsatisfactory quantitative results achieved on an ion trapwere rationalized by high-resolution mass spectrometry. The formation of Mg2-containing cluster isobaric to trimeric cluster [Cu(l-Trp)2Phe] was observed. Interference like this was identified as a possible reason for deterioration of quantitative low-resolution mass spectrometric analyses of real-world samples based on the kinetic method. Cation-exchangerwas used for easy removal of magnesium from a sample and improvement of quantitation. Chiral dependence of formation of the Cu2 containing trimeric clusterwas also observed. Heterochiral diastereoisomeric ions were created less effectively Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2010
Počet záznamů: 1