Počet záznamů: 1

Role of cleavage by separase of the Rec8 kleisin subunit of cohesin during mammalian meiosis I

SYSNO ASEP	0328322
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Role of cleavage by separase of the Rec8 kleisin subunit of cohesin during mammalian meiosis I
Tvůrce(i)	Kudo, Nobuaki R. (AT) Anger, Martin (UZFG-Y)_ORCID Peters, Antoine H. F. M. (AT) Stemmann, O. (DE) Theussl, H. Ch. (AT) Helmhart, W. (AT) Kudo, H. (AT) Heyting, Ch. (NL) Nasmyth, K. (AT)
Zdroj.dok.	Journal of Cell Science. - : Company of Biologists - ISSN 0021-9533 Roč. 122, - (2009), s. 2686-2698
Poč.str.	13 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	Chromosome segregation ; Cohesin ; Meiosis ; Oocyte maturation
Vědní obor RIV	EB - Genetika a molekulární biologie
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000268179400013
DOI	10.1242/jcs.035287
Anotace	Proteolytic activity of separase is required for chiasma resolution during meiosis I in mouse oocytes. Rec8, the meiosis-specific alpha-kleisin subunit of cohesin, is a key target of separase in yeast. Is the equivalent protein also a target in mammals? We show here that separase cleaves mouse Rec8 at three positions in vitro but only when the latter is hyper-phosphorylated. Expression of a Rec8 variant (Rec8-N) that cannot be cleaved in vitro at these sites causes sterility in male mice. Their seminiferous tubules lack a normal complement of 2 C secondary spermatocytes and 1 C spermatids and contain instead a high proportion of cells with enlarged nuclei. Chromosome spreads reveal that Rec8-N expression has no effect in primary spermatocytes but produces secondary spermatocytes and spermatids with a 4 C DNA content, suggesting that the first and possibly also the second meiotic division is abolished. Expression of Rec8-N in oocytes causes chromosome segregation to be asynchronous and delays its completion by 2-3 hours during anaphase I, probably due to inefficient proteolysis of Rec8-N by separase. Despite this effect, chromosome segregation must be quite accurate as Rec8-N does not greatly reduce female fertility. Our data is consistent with the notion that Rec8 cleavage is important and probably crucial for the resolution of chiasmata in males and females.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2010

Počet záznamů: 1