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Glycosidases: a key to tailored carbohydrates
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SYSNO ASEP 0327136 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Glycosidases: a key to tailored carbohydrates Tvůrce(i) Bojarová, Pavla (MBU-M) ORCID
Křen, Vladimír (MBU-M) RID, ORCIDZdroj.dok. Trends in Biotechnology. - : Elsevier - ISSN 0167-7799
Roč. 27, č. 4 (2009), s. 199-209Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova GLYCOSYLTRANSFERASE-CATALYZED SYNTHESIS ; ALPHA-L-ARABINOFURANOSIDASE ; MUTANT GLYCOSIDASES Vědní obor RIV EE - Mikrobiologie, virologie CEP IAA400200503 GA AV ČR - Akademie věd LC06010 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GP203/09/P024 GA ČR - Grantová agentura ČR CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000265136100003 DOI 10.1016/j.tibtech.2008.12.003 Anotace In recent years, carbohydrate-processing enzymes have become the enzymes of choice in many applications thanks to their stereoselectivity and efficiency. This review presents recent developments in glycosidasecatalyzed synthesis via two complementary approaches: the use of wild-type enzymes with engineered substrates, and mutant glycosidases. Genetic engineering has recently produced glucuronyl synthases, an inverting xylosynthase and the first mutant endo-b-N-acetylglucosaminidase. A thorough selection of enzyme strains and aptly modified substrates have resulted in rare glycostructures, such as N-acetyl-b-galactosaminuronates, b1,4-linked mannosides and a1,4-linked galactosides. The efficient selection of mutant enzymes is facilitated by high-throughput screening assays involving the co-expression of coupled enzymes or chemical complementation Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2010
Počet záznamů: 1