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Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system
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SYSNO ASEP 0326146 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system Překlad názvu Vývoj funkčních LH receptorů u prasečích oocyt-kumulárních komplexů kultivovaných in vitro novým modelem dvoustupňové kultivace Tvůrce(i) Procházka, Radek (UZFG-Y) RID, ORCID
Němcová, Lucie (UZFG-Y) RID
Nagyová, Eva (UZFG-Y) RID, ORCID
Scsuková, S. (SK)
Mlynarčíková, A. (SK)Zdroj.dok. Molecular Reproduction and Development - ISSN 1040-452X
Roč. 76, č. 8 (2009), s. 751-761Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova LH Receptors ; oocyte Vědní obor RIV ED - Fyziologie CEP GA523/08/0111 GA ČR - Grantová agentura ČR QG50052 GA MZe - Ministerstvo zemědělství CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000268006400006 DOI 10.1002/mrd.21039 Anotace We show in the present study that freshly isolated pig cumulus-oocyte complexes (COCs) display a limited response to LH, as assessed by the expression of hyaluronan synthase 2 (Has2) mRNA, activation of protein kinase A (PKA), production of hyaluronic acid (HA) and progesterone, cumulus cell expansion and resumption of meiosis. These data indicate that freshly isolated COCs do not possess a sufficient number of functional LH receptors (LHR). However, the expression of Lhr significantly increased during the culture of COCs in vitro in a medium supplemented with FSH. Assuming that the effect of FSH on LHR induction is mediated via cAMP signaling pathways, we developed a new culture system, in which the COCs were pre-cultured for 72 h in a medium supplemented with dbcAMP. The pre-cultured COCs remained in the germinal vesicle stage, their cumulus investment underwent a dramatic increase in size and gap junctions between the cumulus cells were preserved. The stimulation of such COCs with either FSH or LH led to the resumption and completion of meiosis, activation of PKA, expression of Has2, synthesis of large amounts of HA and progesterone, and extensive expansion of cumulus cells. We conclude that the formation of functional LHR is stimulated in cumulus cells during the culture in vitro in a cAMP-dependent pathway. The dbcAMP-treated COCs thus represent a new model in which the resumption of meiosis and cumulus expansion can be induced exclusively by the action of recombinant LH. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2010
Počet záznamů: 1