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Expression of the gene encoding transcription factor PaVP1 differs in Picea abies embryogenic lines depending on their ability to develop somatic embryos
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SYSNO ASEP 0319656 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Expression of the gene encoding transcription factor PaVP1 differs in Picea abies embryogenic lines depending on their ability to develop somatic embryos Překlad názvu Exprese genu kódujícího transkripční faktor PaVP1 se liší u embryogenních linií Picea abies a záleží na jejich schopnosti vyvíjet somatická embrya Tvůrce(i) Fischerová, Lucie (UEB-Q) RID, ORCID
Fischer, L. (CZ)
Vondráková, Zuzana (UEB-Q) RID, ORCID
Vágner, Martin (UEB-Q) RID, ORCIDZdroj.dok. Plant Cell Reports. - : Springer - ISSN 0721-7714
Roč. 27, č. 3 (2008), s. 435-441Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova ABI3/VP1 transcription factor ; Alternative splicing ; Anatomy Vědní obor RIV EB - Genetika a molekulární biologie CEP LN00A081 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LC06034 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy KJB6038402 GA AV ČR - Akademie věd CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000253202100003 DOI 10.1007/s00299-007-0469-6 Anotace Maturation of Norway spruce (Picea abies L.) somatic embryos is induced by abscisic acid (ABA). Several proteins were proven to be involved in ABA sensing including ABI3/VP1 transcription factors and their orthologue PaVP1 was characterized in spruce. To evaluate the role of PaVP1 both in embryogenic potential and in the process of embryo maturation, we studied PaVP1 expression in lines with contrast embryogenic capacities in parallel with detailed anatomical characterization. PaVP1 expression was determined by northern blot hybridisation, which revealed presence of two differentially regulated VP1-like B3-domain transcripts. Full-length PaVP1 transcript level was negligible in all lines on the proliferation media, but it differed strongly on the maturation media containing ABA. In non-embryogenic line, lacking any differentiated structures, the transcript remained undetectable. In contrast, in embryogenic lines with meristematic centres attached to suspensor cells, PaVP1 expression increased strongly after transition onto the maturation media. In highly embryogenic lines, it kept on a high level until the embryos reached cotyledonary stage, while in developmentally arrested line incapable to form mature embryos, the expression dropped down in connection with advanced disintegration of the meristematic centres. Removal of ABA from the maturation media after 2 weeks of maturation resulted in aberrant embryo development and rapid decrease in PaVP1 expression, indicating the impact of exogenously supplemented ABA on both initiation and maintenance of PaVP1 expression and proper embryo development. Since permanently high or increasing PaVP1 transcript levels accompanied proper embryo development in all experiments, it could be regarded as a good marker of this process. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2009
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