Počet záznamů: 1
Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy
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SYSNO ASEP 0314590 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy Překlad názvu Oxidativní fosforylace a energetický status jsou reflektovány morfologií mitochondriální sítě u INS-1E a HEP-G2 buněk zobrazených 4Pi mikroskopií Tvůrce(i) Hlavatá, Lydie (FGU-C)
Lessard, M. (US)
Šantorová, Jitka (FGU-C) RID
Bewersdorf, J. (US)
Ježek, Petr (FGU-C) RID, ORCIDZdroj.dok. Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728
Roč. 1777, 7-8 (2008), s. 834-846Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova 4Pi microscopy ; 100 nm 3D imaging ; mitochondrial network Vědní obor RIV EA - Morfologické obory a cytologie CEP NR7917 GA MZd - Ministerstvo zdravotnictví NR9183 GA MZd - Ministerstvo zdravotnictví IAA500110701 GA AV ČR - Akademie věd GP303/05/P100 GA ČR - Grantová agentura ČR CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000257696400039 DOI 10.1016/j.bbabio.2008.04.002 Anotace Using 4Pi microscopy, 3D morphology changes of mitochondrial reticulum network responding to decreased oxidative phosphorylation have been observed in INS-1E and HEP-G2 cells at an unprecedented 100 nm level of detail. In INS-1E cells a single interconnected mitochondrion with a nearly constant 262+26 nm tubule diameter was found. With rotenone inhibiting respiration to 10%, disintegration into several reticula and numerous 300 nm spheres or short tubules was observed. De-energization by uncoupling additionally led to formation of rings and bulky cisternae of 1.4+0.4 μm diameter. Rotenone and uncoupler acted synergically in INS-1E cells and increased fusion (ongoing with fission) forming bowl-like shapes. In HEP-G2 cells fission partially ceased with FCCP plus rotenone. Thus we have revealed previously undescribed details for shapes upon mitochondrial disintegration and clearly demonstrate that high resolution 3D microscopy is required for visualization of mitochondrial network Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2009
Počet záznamů: 1