Počet záznamů: 1

LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147

SYSNO ASEP	0307370
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147
Překlad názvu	LFA-1-zprostředkovaná adheze leukocytů je regulována interakcemi CD43 s LFA-1 a CD147
Tvůrce(i)	Khunkaewla, P. (AT) Schiller, H.B. (AT) Paster, W. (AT) Leksa, V. (SK) Čermák, Lukáš (UMG-J)_ORCID Anděra, Ladislav (UMG-J)_RID Hořejší, Václav (UMG-J)_RID Stockinger, H. (AT)
Zdroj.dok.	Molecular Immunology. - : Elsevier - ISSN 0161-5890 Roč. 45, č. 6 (2008), s. 1703-1711
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	leukocyte adhesion and aggregation ; monoclonal antibodies ; receptor signaling
Vědní obor RIV	EB - Genetika a molekulární biologie
CEP	1M0506 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000254181800017
Anotace	Activity of LFA-1 (CD11a/CD18) must be tightly controlled at onset of cellular immunity. Sialoglycoprotein CD43 can affect LFA-1-mediated cell adhesion in anti- or pro-adhesive manner. Using Y2H screen and coimmunoprecipitation we identified and characterized physical and functional association of CD43 with 2 novel partners, LFA-1 and CD147. Monoclonal antibodies to both CD43 and CD147 induced homotypic cell aggregation and adhesion of Jurkat T cells and U937 myeloid cells as well as dynamic co-capping of LFA-1 together with CD43 and CD147 to cell contact zones. Cotransfection of CD43 interfered with CD147-induced cell adhesion and aggregation, and siRNA-mediated knock down of CD43 in human T cells led to enhanced LFA-1 activation induced via CD147 and T cell antigen receptor. Triggering CD43 and underlying signaling pathways thus seems to enhance LFA-1 adhesiveness. CD43 also negatively regulates LFA-1 induction via other receptors by dynamic interaction with either LFA-1 or CD147.
Pracoviště	Ústav molekulární genetiky
Kontakt	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Rok sběru	2009

Počet záznamů: 1