Počet záznamů: 1
Molecular identification of three crested newt species (Triturus cristatus superspecies) by RAPD markers
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SYSNO ASEP 0164544 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Molecular identification of three crested newt species (Triturus cristatus superspecies) by RAPD markers Tvůrce(i) Mikulíček, P. (CZ)
Piálek, Jaroslav (UBO-W) RID, ORCID, SAIZdroj.dok. Amphibia-Reptilia. - : Brill - ISSN 0173-5373
Roč. 24, č. 2 (2003), s. 201-207Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Triturus cristatus ; genetic variation Vědní obor RIV EB - Genetika a molekulární biologie CEP GA206/98/0115 GA ČR - Grantová agentura ČR GA206/01/0695 GA ČR - Grantová agentura ČR KSK6005114 GA AV ČR - Akademie věd CEZ AV0Z6093917 - UBO-W MSM 113100004 DOI 10.1163/156853803322390444 Anotace We have applied Random amplified polymorphic DNA (RAPD) analysis to three newt species T. cristatus, T. dobrogicus and T. carnifex occurring in the Czech Republic and Slovakia to identify species-specific molecular markers, potentially useful for newt determination. Out of twenty randomly used RAPD primers, just one did not amplify any DNA fragment. Amplification reactions from the other 19 primers yielded 2 to 22 bands per primer. The total number of scored bands was 202. The average number of bands per primer and individual was 5.3. While the similarity indexes among individuals within populations (0.94 ů 0.99) and between populations within species (0.81 ů 0.98) were very high, between-species similarity was relatively low with the lowest value for T. carnifex and T. cristatus (0.49) and the highest value for T. dobrogicus and T. cristatus (0.53). Although nineteen primers amplified DNA fragments, only twelve generated clear highly reproducible species-specific bands. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2004
Počet záznamů: 1