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Microbiotests of cyanobacterial toxins screening

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    SYSNO ASEP0129231
    Druh ASEPC - Konferenční příspěvek (mezinárodní konf.)
    Zařazení RIVD - Článek ve sborníku
    NázevMicrobiotests of cyanobacterial toxins screening
    Tvůrce(i) Maršálek, Blahoslav (BU-J) RID, ORCID
    Bláha, L. (CZ)
    Zdroj.dok.New microbiotests for routine toxicity screening and biomonitoring / Persoone G. ; Janssen C. ; De Coen W.. - New York : Kluwer Academic / Plenum Publishers, 2000 - ISBN 0-306-46406-3
    Rozsah strans. 519-525
    Poč.str.7 s.
    AkceInternational Symposium New Microbiotests for Routine Toxicity Screening and Biomonitoring
    Datum konání01.06.1998-03.06.1998
    Místo konáníBrno
    ZeměCZ - Česká republika
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovatoxicity. ; microbiotests. ; cyanobacteria.
    Vědní obor RIVEF - Botanika
    CEPKSK2005601 GA AV ČR - Akademie věd
    AnotaceMicrobioassays are typical non-specific tests. The toxicity signal results from the whole spectrum of consequences, especially, when phytoplankton biomass is tested for toxins. Embryotoxicity, genotoxicity and mutagenity, immunotoxicity ,cytotoxicity and other toxic cyanobacterial metabolites can be tested by specific miniaturised biosystems. Typical problems occur when general ecotoxicity tests are used for the pre-screening of cyanotoxins. Bacterial tests were found as quite unreliable because no correlation with dose response of main toxins was found. Extracts of cyanobacterial biomass provide a number of interpretation problems also for crustacean and protozoan species, which were found to be sensitive for cyanotoxins. We developed system for the fractionation of biomass. The fraction of toxins, pigments and other metabolites are separed by C-18 cartridges and molecular sieves (10.000MV) and are tested by biological assays. Mortality of Thamnocephalus or Daphnia in crude cyanobacterial extracts can be caused for example by the lack of oxygen or other factors which have no consequences with the cyanotoxins. However, microbiotests using fractionated biomass can be used as pre-screening of cyanotoxins in drinking water supply or recreational water bodies.Microbiotests for cyanotoxins toxicity pre-screening.
    PracovištěBotanický ústav
    KontaktMartina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8
    Rok sběru2002

Počet záznamů: 1