Počet záznamů: 1
Changes in cytokinin and auxin concentrations in seaweed concentrates when stored at an elevated temperature
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SYSNO ASEP 0109756 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Changes in cytokinin and auxin concentrations in seaweed concentrates when stored at an elevated temperature Překlad názvu Changes in cytokinin and auxin concentrations in seaweed concentrates when stored at an elevated temperature Tvůrce(i) Stirk, W. A. (ZA)
Arthur, G. D. (ZA)
Lourens, A. F. (ZA)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Strnad, Miroslav (UEB-Q) RID, ORCID
van Staden, J. (ZA)Zdroj.dok. Journal of Phycology. - : Wiley - ISSN 0022-3646
Roč. 16, č. 1 (2004), s. 31-39Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova accelerated storage ; auxins ; cytokinins Vědní obor RIV EF - Botanika CEP GA522/03/0323 GA ČR - Grantová agentura ČR CEZ AV0Z5038910 - UEB-Q Anotace Two seaweed concentrates were made from the kelps Ecklonia maxima and Macrocystis pyrifera using a cell burst method. Cytokinin- and auxin-like activities were measured using the soybean callus and mungbean bioassays, respectively. Cytokinin-like activity was detected in both seaweed concentrates, being equivalent to approximately 50 μg L-1 kinetin. Auxin-like activity was also detected in both concentrates with the E. maxima derived concentrate having higher biological activity, equivalent to 10-5ů10-4 M indole-butyric acid. Two replicates of each concentrate were stored at 54 °C for 14 days to accelerate the effects of storage. Both fresh and stored samples of the two seaweed concentrates were analysed for their endogenous cytokinin and auxin content. The samples were purified using a combined DEAE-Sephadex octadecylsilica column and immunoaffinity chromatography based on wide-range cytokinin and IAA specific monoclonal antibodies. These extracts were analysed by HPLC linked to a Micromass single quadrupole mass spectrophotometer. Eighteen and nineteen different cytokinins were detected, respectively, in the two concentrates, with trans-zeatin-O-glucoside being the main cytokinin present. Accelerated storage of the concentrates caused an increase in the total cytokinin concentration with a large increase in the aromatic meta-topolin. Indole-3-acetic acid was the main auxin in both seaweed concentrates. Indole conjugates, including amino acid conjugates, were also quantified. The total auxin concentration decreased with accelerated storage for both concentrates. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2005
Počet záznamů: 1