Počet záznamů: 1
A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples
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SYSNO ASEP 0084909 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve SCOPUS Název A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples Překlad názvu Rychlý PCR-test na druhovou identifikaci dvou kryptických druhů netopýrů Pipistrellus pipistrellus a P. pygmaeus a jeho aplikace na muzejní vzorky a vzorky trusu Tvůrce(i) Kaňuch, Peter (UBO-W)
Hájková, Petra (UBO-W) RID, ORCID
Řehák, Z. (CZ)
Bryja, Josef (UBO-W) RID, SAI, ORCIDZdroj.dok. Acta Chiropterologica. - : Polska Akademia Nauk - ISSN 1508-1109
Roč. 9, č. 1 (2007), s. 277-282Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova bats ; cytochrome b ; mtDNA ; sibling species ; non-invasive sampling Vědní obor RIV EB - Genetika a molekulární biologie CEP GA206/06/0954 GA ČR - Grantová agentura ČR CEZ AV0Z60930519 - UBO-W (2005-2011) EID SCOPUS 34548505059 DOI 10.3161/1733-5329(2007)9[277:ARPTFS]2.0.CO;2 Anotace The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2008
Počet záznamů: 1