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Changes in nonpolar aldehydes in bean cotyledons during ageing
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SYSNO ASEP 0079980 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Changes in nonpolar aldehydes in bean cotyledons during ageing Překlad názvu Změny v nepolárních aldehydech v děložních listech fazolu během stárnutí Tvůrce(i) Wilhelmová, Naděžda (UEB-Q) RID, ORCID
Domingues, P. (PT)
Srbová, M. (CZ)
Fuksová, H. (CZ)
Wilhelm, J. (CZ)Zdroj.dok. Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134
Roč. 50, č. 4 (2006), s. 559-564Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Ageing ; aldehydes ; lipid peroxidation ; lipofuscin-like pigments (LFP) Vědní obor RIV CE - Biochemie CEP GA522/03/0312 GA ČR - Grantová agentura ČR CEZ AV0Z50380511 - UEB-Q (2005-2011) Anotace Ageing of plant organs is accompanied by an increased production of free radicals what results in membrane lipid peroxidation. Non-polar aldehydes originating from this process interact with the cellular material to form the fluorescent end-products, lipofuscin-like pigments (LFP). Their formation was studied both qualitatively and quantitatively in ageing of bean cotyledons. The concentration of lipofuscin-like pigments increased 9-fold in 14-d-old (senescent) cotyledons in relation to 8-d-old (young) cotyledons. HPLC fractionation patterns indicate changes in their composition during ageing. The LFP increase in old cotyledons was accompanied by elevated levels of non-polar aldehydes that increased during ageing to 167 %. The composition of aldehydes was studied by mass spectrometry. The most abundant fraction in both young and old cotyledon was represented by C12 aldehydes, which comprised both saturated and unsaturated species. We have observed differences in abundances of individual aldehydes between the young and the old cotyledons that might explain the differences in the composition of lipofuscin-like pigments. These results support the involvement of free radicals in plant ageing; however, it is suggested that plant aldehydic products of lipid peroxidation differ from those found in animals. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2007
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