Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae
1.
SYSNO ASEP
0040292
Druh ASEP
J - Článek v odborném periodiku
Zařazení RIV
J - Článek v odborném periodiku
Poddruh J
Ostatní články
Název
Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae
Překlad názvu
Kompartmentace rostlinného transporteru založená na lipidech
Tvůrce(i)
Grossmann, Q. (DE) Opekarová, Miroslava (MBU-M) Nováková, L. (DE) Stolz, J. (DE) Tanner, W. (DE)
Zdroj.dok.
Eukaryotic Cell
- ISSN 1535-9778
Roč. 5, č. 6 (2006), s. 945-953
Poč.str.
9 s.
Jazyk dok.
eng - angličtina
Země vyd.
US - Spojené státy americké
Klíč. slova
saccharomyces cerevisiae ; plant transport protein ; hup1
Vědní obor RIV
EE - Mikrobiologie, virologie
CEP
LC545 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
CEZ
AV0Z50200510 - MBU-M (2005-2011)
Anotace
The hexose-proton symporter HUP1 shows a spotty distribution in the plasma membrane of the green alga Chlorella kessleri. Chlorella cannot be transformed so far. To study the membrane localization of the HUP1 protein in detail, the symporter was fused to green fluorescent protein (GFP) and heterologously expressed in Saccharomyces cerevisiae and Schizosaccharomyces pombe. In these organisms, the HUP1 protein has previously been shown to be fully active. The GFP fusion protein was exclusively targeted to the plasma membranes of both types of fungal cells. In S. cerevisiae, it was distributed nonhomogenously and concentrated in spots resembling the patchy appearance observed previously for endogenous H(+) symporters. It is documented that the Chlorella protein colocalizes with yeast proteins that are concentrated in 300-nm raft-based membrane compartments
