Počet záznamů: 1
Comparative phenotypic characterization of keratinocytes originating from hair follicles
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SYSNO ASEP 0029197 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Comparative phenotypic characterization of keratinocytes originating from hair follicles Překlad názvu Srovnávací fenotypová charakterizace keratinocytů pocházejících z vlasových folikulů Tvůrce(i) Klíma, Jiří (UZFG-Y) RID, ORCID
Smetana Jr., K. (CZ)
Motlík, Jan (UZFG-Y) RID, ORCID
Plzáková, Z. (CZ)
Liu, F. T. (US)
Štork, J. (CZ)
Kaltner, H. (DE)
Chovanec, M. (CZ)
Dvořánková, B. (CZ)
André, S. (DE)
Gabius, H. J. (DE)Zdroj.dok. Journal of Molecular Histology
Roč. 36, - (2005), s. 89-96Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova hair follicles Vědní obor RIV EB - Genetika a molekulární biologie CEP LN00A065 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy IBS4050005 GA AV ČR - Akademie věd GA304/04/0171 GA ČR - Grantová agentura ČR CEZ AV0Z50450515 - UZFG-Y (2005-2011) Anotace The principal pool of epidermal stem cells is located in the bulge region of the hair follicle root sheath. In this research project, we have used a refined procedure to isolate porcine hair follicles including their root sheath and for comparison purposes also human cell material. These cells migrating from the hair follicles were then cytochemically characterized. A panel of antibodies and two labeled plant lectins were tested on cell material obtained under a range of assorted experimental conditions. Due to their role in growth regulation we also studied two endogenous lectins, specifically monitoring their expression and the presence of accessible ligands. These in vitro results were compared with findings on porcine and human hair follicles and human basal cell carcinomas in situ. The keratinocytes originating from hair follicles in the presence of feeder cells are rather undifferentiated and express galectin-1/galectin-1-binding sites but not galectin-3 in their nuclei associated with DeltaNp63alpha positivity. Nuclear reactivity for galectin-1 was rarely observed in the bulge of the outer root sheath of the human hair follicle and of basal cell carcinomas and absent in porcine tissue samples. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2006
Počet záznamů: 1