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The Trypanosoma brucei La protein is a candidate poly(U) shield that impacts spliced leader RNA maturation and tRNA intron removal
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SYSNO ASEP 0023737 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název The Trypanosoma brucei La protein is a candidate poly(U) shield that impacts spliced leader RNA maturation and tRNA intron removal Překlad názvu La protein Trypanosoma brucei je kandidátní poly(U) chránící protein, který ovlivňuje zrání spliced leader RNA a odstranění intronu z tRNA Tvůrce(i) Trantírková, Silvie (PAU-O) RID
Paris, Zdeněk (PAU-O)
Sturm, N. R. (US)
Campbell, D. A. (US)
Lukeš, Julius (PAU-O)Zdroj.dok. International Journal for Parasitology. - : Elsevier - ISSN 0020-7519
Roč. 35, č. 4 (2005), s. 359-366Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. AU - Austrálie Klíč. slova splicing ; Trypanosoma ; RNA interference Vědní obor RIV EB - Genetika a molekulární biologie CEP IAA5022302 GA AV ČR - Akademie věd CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) Anotace By virtue of its preferential binding to poly(U) tails on small RNA precursors and nuclear localisation motif, the La protein has been implicated for a role in the stabilisation and nuclear retention of processing intermediates for a variety of small RNAs in eukaryotic cells. La protein was targeted for selective knockdown by inducible RNA interference in Trypanosoma brucei. In the relative absence of La protein T. brucei cells were not viable, in contrast to La gene knockouts in yeast. A variety of potential small RNA substrates were examined under induction, including spliced leader RNA, spliced leader associated RNA, the U1, U2, U4, and U6 small nuclear RNAs, 5S ribosomal RNA, U3 small nucleolar RNA, and tRNATyr. This study indicates that La protein binding is not essential for maturation of the spliced leader RNA, but does not rule out the presence of an alternative processing pathway that could compensate for the absence of normally-associated La protein. Pracoviště Parazitologický ústav (do r. 2005) Kontakt Kateřina Homrová,katerina.homrova@paru.cas.cz , Tel.: 387 775 406 Rok sběru 2006
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