The effect of peptides and proteins from cyanobacterium Microcystis aeruginosa on coagulation of kaolin particles

0378897 - ÚH 2013 HU eng C - Konferenční příspěvek (zahraniční konf.)
Šafaříková, Jana - Pivokonský, Martin
The effect of peptides and proteins from cyanobacterium Microcystis aeruginosa on coagulation of kaolin particles.
Proceedings of the 6th IWA International Conference for Young Water Professionals. Budapest: IWA, 2012, IWA-9893.
[IWA International Conference for Young Water Professionals /6./ (IWA YWPC 2012). Budapest (HU), 10.07.2012-13.07.2012]
Grant CEP: GA ČR GAP105/11/0247
Výzkumný záměr: CEZ:AV0Z20600510
Klíčová slova: algal organic matter (AOM) * coagulation * complexes formation * peptides/proteins
Kód oboru RIV: BK - Mechanika tekutin

In this study, the coagulation of peptides and proteins produced by cyanobacterium Microcystis aeruginosa and their influence on the coagulation of hydrophobic kaolin particles were investigated. For this purpose, the dosage of ferric sulphate used as coagulant was optimized and jar tests with kaolin, peptides/proteins and both kaolin and peptides/proteins were carried out under different pH conditions. At pH 4 – 5.5 the efficient coagulation of peptides/proteins took place and peptides/proteins were also found to contribute to the coagulation of kaolin particles at this pH. Charge neutralization and adsorption were found to be dominant coagulation mechanisms. The coagulation efficiency and the character of the prevailing coagulation mechanism were strongly dependent on charge characteristics of peptides/proteins, kaolin and hydrolysis products of iron used as coagulation agent. At pH about 6, the coagulation process was deteriorated by the formation of soluble Fe-peptide/protein complexes. Through affinity chromatography analysis it was demonstrated that peptides/proteins able to form complexes with iron are of MW 52, 10, 8.4, 7.7, 6.5, 2.8 and 1 kDa.
Trvalý link: http://hdl.handle.net/11104/0210218