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Excess ischemic tachyarrhythmias trigger protection infarction in rats

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    0546478 - FGÚ 2022 RIV GB eng J - Článek v odborném periodiku
    Neckář, Jan - Alánová, Petra - Olejníčková, Veronika - Papoušek, František - Hejnová, L. - Šilhavý, Jan - Behuliak, Michal - Bencze, Michal - Hrdlička, Jaroslav - Vecka, M. - Jarkovská, D. - Švíglerová, J. - Mistrová, E. - Štengl, M. - Novotný, J. - Ošťádal, Bohuslav - Pravenec, Michal - Kolář, František
    Excess ischemic tachyarrhythmias trigger protection infarction in rats.
    Clinical science. Roč. 135, č. 17 (2021), s. 2143-2163. ISSN 0143-5221. E-ISSN 1470-8736
    Grant CEP: GA ČR(CZ) GA18-03207S
    Grant ostatní: AV ČR(CZ) AP1502
    Program: Akademická prémie - Praemium Academiae
    Institucionální podpora: RVO:67985823
    Klíčová slova: C-reactive protein * heart * metabolomics * myocardial infarction * remote ischemic perconditioning * ventricular arrhythmias
    Obor OECD: Physiology (including cytology)
    Impakt faktor: 6.876, rok: 2021
    Způsob publikování: Omezený přístup
    https://doi.org/10.1042/CS20210648

    Increased level of C-reactive protein (CRP) is a risk factor for cardiovascular diseases, including myocardial infarction and hypertension. Here, we analyzed the effects of CRP overexpression on cardiac susceptibility to ischemia/reperfusion (I/R) injury in adult spontaneously hypertensive rats (SHR) expressing human CRP transgene (SHR-CRP). Using an in vivo model of coronary artery occlusion, we found that transgenic expression of CRP predisposed SHR-CRP to repeated and prolonged ventricular tachyarrhythmias. Excessive ischemic arrhythmias in SHR-CRP led to a significant reduction in infarct size (IS) compared with SHR. The proarrhythmic phenotype in SHR-CRP was associated with altered heart and plasma eicosanoids, myocardial composition of fatty acids (FAs) in phospholipids, and autonomic nervous system imbalance before ischemia. To explain unexpected IS-limiting effect in SHR-CRP, we performed metabolomic analysis of plasma before and after ischemia. We also determined cardiac ischemic tolerance in hearts subjected to remote ischemic perconditioning (RIPer) and in hearts ex vivo. Acute ischemia in SHR-CRP markedly increased plasma levels of multiple potent cardioprotective molecules that could reduce IS at reperfusion. RIPer provided IS-limiting effect in SHR that was comparable with myocardial infarction observed in naïve SHR-CRP. In hearts ex vivo, IS did not differ between the strains, suggesting that extra-cardiac factors play a crucial role in protection. Our study shows that transgenic expression of human CRP predisposes SHR-CRP to excess ischemic ventricular tachyarrhythmias associated with a drop of pump function that triggers myocardial salvage against lethal I/R injury likely mediated by protective substances released to blood from hypoxic organs and tissue at reperfusion.
    Trvalý link: http://hdl.handle.net/11104/0322978

     
     
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