Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration

0543905 - FGÚ 2022 RIV NL eng J - Článek v odborném periodiku
Dauss, E. - Papoušková, Klára - Sychrová, Hana - Rapoport, A.
Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration.
Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Roč. 114, č. 7 (2021), s. 1069-1077. ISSN 0003-6072. E-ISSN 1572-9699
Grant CEP: GA MŠMT(CZ) LTC20006
Grant ostatní: COST (European Cooperation in Science and Technology)(XE) CA18113
Institucionální podpora: RVO:67985823
Klíčová slova: anhydrobiosis * dehydration-rehydration * yeast * endoplasmic reticulum * endoplasmic reticulum protein Ist2
Obor OECD: Microbiology
Impakt faktor: 2.158, rok: 2021
Způsob publikování: Omezený přístup
https://doi.org/10.1007/s10482-021-01578-8

Two Saccharomyces cerevisiae strains, BY4741 and BY4741-derived strain lacking the IST2 gene (ist2 Delta), were used to characterise the possible role of cortical endoplasmic reticulum (ER) protein Ist2 upon cell dehydration and subsequent rehydration. For the first time, we show that not only protein components of the plasma membrane (PM), but also at least one ER membrane protein (Ist2) play an important role in the maintenance of the viability of yeast cells during dehydration and subsequent rehydration. The low viability of the mutant strain ist2 increment upon dehydration-rehydration stress was related to the lack of Ist2 protein in the ER. We revealed that the PM of ist2 increment strain is not able to completely restore its molecular organisation during reactivation from the state of anhydrobiosis. As the result, the permeability of the PM remains high regardless of the type of reactivation (rapid or gradual rehydration). We conclude that ER protein Ist2 plays an important role in ensuring the stability of molecular organisation and functionality of the PM during dehydration-rehydration stress. These results indicate an important role of ER-PM interactions during cells transition into the state of anhydrobiosis and the subsequent restoration of their physiological activities.
Trvalý link: http://hdl.handle.net/11104/0321010