Gradual cold acclimation induces cardioprotection without affecting adrenergic beta-receptor-mediated adenylyl cyclase signaling

0524244 - FGÚ 2021 RIV US eng J - Článek v odborném periodiku
Tibenská, V. - Benešová, A. - Vebr, P. - Liptáková, A. - Hejnová, L. - Elsnicová, B. - Drahota, Zdeněk - Horníková, D. - Galatík, F. - Kolář, D. - Vybíral, S. - Alánová, Petra - Novotný, J. - Kolář, František - Nováková, Olga - Žurmanová, J.M.
Gradual cold acclimation induces cardioprotection without affecting adrenergic beta-receptor-mediated adenylyl cyclase signaling.
Journal of Applied Physiology. Roč. 128, č. 4 (2020), s. 1023-1032. ISSN 8750-7587. E-ISSN 1522-1601
Grant CEP: GA ČR GA17-07748S
Institucionální podpora: RVO:67985823
Klíčová slova: adenylyl cyclase * beta-adrenergic receptors * cardioprotection * cold acclimation * mitochondria
Obor OECD: Physiology (including cytology)
Impakt faktor: 3.532, rok: 2020
Způsob publikování: Omezený přístup
https://doi.org/10.1152/japplphysiol.00511.2019

Novel strategies are needed that can stimulate endogenous signaling pathways to protect the heart from myocardial infarction. The present study tested the hypothesis that appropriate regimen of cold acclimation (CA) may provide a promising approach for improving myocardial resistance to ischemia/reperfusion (UR) injury without negative side effects. We evaluated myocardial UR injury, mitochondrial swelling, and beta-adrenergic receptor (beta-AR)-adenylyl cyclase-mediated signaling. Male Wistar rats were exposed to CA (8 degrees C, 8 h/day for a week, followed by 4 wk at 8 degrees C for 24 h/day), while the recovery group (CAR) was kept at 24 degrees C for an additional 2 wk. The myocardial infarction induced by coronary occlusion for 20 min followed by 3-h reperfusion was reduced from 56% in controls to 30% and 23% after CA and CAR. respectively. In line, the rate of mitochondrial swelling at 200 mu M Ca2+ was decreased in both groups. Acute administration of metoprolol decreased infarction in control group and did not affect the CA-elicited cardiprotection. Accordingly, neither beta 1-AR-G(s)alpha-adenyly-1- cyclase signaling. stimulated with specific ligands, nor p-PKA/PICA ratios were affected after CA or CAR. Importantly. Western blot and immunofluorescence analyses revealed beta 2- and beta 3-AR protein enrichment in membranes in both experimental groups. We conclude that gradual cold acclimation results in a persisting increase of myocardial resistance to I/R injury without hypertension and hypertrophy. The cardioprotective phenotype is associated with unaltered adenylyl cyclase signaling and increased mitochondrial resistance to Ca2+-overload. The potential role of upregulated beta 2/beta 3-AR pathways remains to be elucidated.
Trvalý link: http://hdl.handle.net/11104/0308629