PLK1 regulates spindle formation kinetics and APC/C activation in mouse zygote

0460308 - ÚŽFG 2017 RIV GB eng J - Článek v odborném periodiku
Baran, V. - Brzáková, Adéla - Rehák, P. - Kovaříková, V. - Šolc, Petr
PLK1 regulates spindle formation kinetics and APC/C activation in mouse zygote.
Zygote. Roč. 24, č. 3 (2016), s. 338-345. ISSN 0967-1994. E-ISSN 1469-8730
Grant CEP: GA MŠMT ED2.1.00/03.0124; GA MŠMT LH12057
Institucionální podpora: RVO:67985904
Klíčová slova: APC/C * BI2536 * live cell imaging * mouse zygote * PLK1 * securin * spindle assembly
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.053, rok: 2016

Polo-like kinase 1 (PLK1) is involved in essential events of cell cycle including mitosis in which it participates in centrosomal microtubule nucleation, spindle bipolarity establishment and cytokinesis. Although PLK1 function has been studied in cycling cancer cells, only limited data are known about its role in the first mitosis of mammalian zygotes. During the 1-cell stage of mouse embryo development, the acentriolar spindle is formed and the shift from acentriolar to centrosomal spindle formation progresses gradually throughout the preimplantation stage, thus providing a unique possibility to study acentriolar spindle formation. We have shown previously that PLK1 activity is not essential for entry into first mitosis, but is required for correct spindle formation and anaphase onset in 1-cell mouse embryos. In the present study, we extend this knowledge by employing quantitative confocal live cell imaging to determine spindle formation kinetics in the absence of PLK1 activity and answer the question whether metaphase arrest at PLK1-inhibited embryos is associated with low anaphase-promoting complex/cyclosome (APC/C) activity and consequently high securin level. We have shown that inhibition of PLK1 activity induces a delay in onset of acentriolar spindle formation during first mitosis. Although these PLK1-inhibited 1-cell embryos were finally able to form a bipolar spindle, not all chromosomes were aligned at the metaphase equator. PLK1-inhibited embryos were arrested in metaphase without any sign of APC/C activation with high securin levels. Our results document that PLK1 controls the onset of spindle assembly and spindle formation, and is essential for APC/C activation before anaphase onset in mouse zygotes.
Trvalý link: http://hdl.handle.net/11104/0260423