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LBP/BPI homologue in Eisenia andrei earthworms

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    0460102 - MBÚ 2017 RIV GB eng J - Článek v odborném periodiku
    Škanta, František - Procházková, Petra - Roubalová, Radka - Dvořák, Jiří - Bilej, Martin
    LBP/BPI homologue in Eisenia andrei earthworms.
    Developmental and Comparative Immunology. Roč. 54, č. 1 (2016), s. 1-6. ISSN 0145-305X. E-ISSN 1879-0089
    Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) EE2.3.30.0003
    Institucionální podpora: RVO:61388971
    Klíčová slova: Earthworm * Eisenia * LBP/BPI
    Kód oboru RIV: EC - Imunologie
    Impakt faktor: 3.218, rok: 2016

    LBP/BPIs are pattern recognition receptors that are often present in vertebrates and in invertebrates, and they play a defense role against pathogens. We have identified 1698 bp cDNA sequence from the Eisenia andrei earthworm with predicted amino acid sequence that shares homology with the LBP/BPI family (EaLBP/BPI). Sequence analysis of EaLBP/BPI proved the existence of two conserved domains with the potential ability to bind LPS. The predicted molecular mass of the EaLBP/BPI protein is 53.5 kDa, and its high basicity (pI 9.8) is caused by its high arginine content. Constitutive transcription of the Ealbp/bpi gene was shown in all tested tissues, with the highest level in coelomocytes and seminal vesicles; the lowest level was detected in the intestine. On the contrary, another earthworm LPS-binding molecule CCF (coelomic cytolytic factor) was expressed only in the intestine and coelomocytes. In E. andrei coelomocytes, the transcription of Ealbp/bpi gene was up-regulated in response to bacterial stimulation, reaching a maximum at 8 and 16 h post stimulation with Bacillus subtilis and Escherichia coli, respectively.
    Trvalý link: http://hdl.handle.net/11104/0260241

     
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