Early identification of etiological agents of fungal infection and assessment of susceptibility antifungal drug, amphotericin B, by advanced separation techniques in human blood

0451034 - ÚIACH 2016 GR eng A - Abstrakt
Horká, Marie - Šlais, Karel - Šalplachta, Jiří - Růžička, F. - Tesařová, Marie - Karásek, Pavel - Roth, Michal
Early identification of etiological agents of fungal infection and assessment of susceptibility antifungal drug, amphotericin B, by advanced separation techniques in human blood.
IMA 2015. Book of abstracts. 2015. s. 206-206.
[International Conference on Instrumental methods of Analysis: Modern Trends and Applications /9./. 20.09.2015-24.09.2015, Kalamata]
Grant CEP: GA MV VG20112015021; GA MV VG20102015023
Institucionální podpora: RVO:68081715
Klíčová slova: electrophoretic techniques and MALDI-TOF MS * amphotericin B * Candida species
Kód oboru RIV: CB - Analytická chemie, separace

The pre-concentration of fungal pathogens, and amphotericin B (0.3 ng mL-1) from blood on segmented strip, and their subsequent CZE, CIEF (UV detection) were tested as fast and low-cost methods to the detection of these analytes in FS capillaries etched with supercritical water and modified with (3-glycidyloxypropyl)trimethoxysilane. Simultaneously, the differences in the chemotaxonomy fingerprints of the microorganisms can be utilized by MALDI-TOF MS.
Trvalý link: http://hdl.handle.net/11104/0252215