Single cell analysis of signaling molecules

0437862 - ÚIACH 2015 BR eng A - Abstrakt
Klepárník, Karel - Luksch, Jaroslav - Adamová, Eva - Potáčová, Anna - Matalová, E. - Foret, František
Single cell analysis of signaling molecules.
ITP & LACE 2014. Book of Abstracts. Grupo VLS Print Solution, 2014 - (Guzman, N.; Taveres, M.). s. 49-49
[International Symposium on Electro- and Liquid Phase-Separation Techniques /21./ and Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology /21./. 04.10.2014-08.10.2014, Natal]
Grant CEP: GA ČR(CZ) GA14-28254S
Institucionální podpora: RVO:68081715 ; RVO:67985904
Klíčová slova: single cell analysis * signaling molecules * caspase
Kód oboru RIV: CB - Analytická chemie, separace

We have synthesized a probe consisting of CdTe QDs conjugated with oligonucleotide to be used as a donor. Conjugation reaction between carboxylated QDs and aminated oligonucleotides has been performed via zero-length cross-linkers. Sample consisting of clinically-relevant PCR amplicons labelled with ROX (6-carboxyrhodamine) served as acceptor. Upon mutation-specific sample-probe hybridization an increase in fluorescence intensity at 610 nm (the emission spectra maximum of ROX) confirmed a proper function of FRET at a presence of detected mutation. The limit of detection evaluated for PCR amplified fragments of the sample is 4x10-9 M.
Trvalý link: http://hdl.handle.net/11104/0241334