Nonhomologous DNA end joining and chromosome aberrations in human embryonic lung fibroblasts treated with environmental pollutants

0435644 - ÚEM 2015 RIV NL eng J - Článek v odborném periodiku
Rössner ml., Pavel - Rössnerová, Andrea - Beskid, Olena - Tabashidze, Nana - Líbalová, Helena - Uhlířová, Kateřina - Topinka, Jan - Šrám, Radim
Nonhomologous DNA end joining and chromosome aberrations in human embryonic lung fibroblasts treated with environmental pollutants.
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 763-764, MAY-JUN 2014 (2014), s. 28-38. ISSN 0027-5107
Grant CEP: GA ČR GAP503/11/0084
Institucionální podpora: RVO:68378041
Klíčová slova: benzo[a]pyrene * chromosome aberrations * double-strand DNA breaks
Kód oboru RIV: DN - Vliv životního prostředí na zdraví
Impakt faktor: 3.680, rok: 2014

The cells were treated for 24 h with B[a]P (1 μM, 10 μM, 25 μM) and EOMs (1 μg/ml, 10 μg/ml, 25 μg/ml). 25 μM of B[a]P and most of the tested doses of EOMs induced double strand breaks (DSBs) as indicated by H2AX phosphorylation. DNA damage was accompanied by induction of XRCC4 expression and an increased frequency of chromosomal aberrations. Translocations most frequently affected chromosome 7. We observed only a weak induction of Ku70/80 expression as well as ligation activity of nuclear extracts.
Trvalý link: http://hdl.handle.net/11104/0239451