Nuclear track-based biosensors with the enzyme laccase

0432984 - ÚJF 2015 RIV NL eng J - Článek v odborném periodiku
Garcia-Arellano, H. - Fink, Dietmar - Hernandez, G. M. - Vacík, Jiří - Hnatowicz, Vladimír - Alfonta, L.
Nuclear track-based biosensors with the enzyme laccase.
Applied Surface Science. Roč. 310, SI (2014), s. 66-76. ISSN 0169-4332. E-ISSN 1873-5584
Grant CEP: GA ČR(CZ) GBP108/12/G108; GA MŠMT(XE) LM2011019
Institucionální podpora: RVO:61389005
Klíčová slova: Biosensor * Laaccase * nuclear tracks * Phenolic compounds
Kód oboru RIV: BM - Fyzika pevných látek a magnetismus
Impakt faktor: 2.711, rok: 2014

A new type of biosensors for detecting phenolic compounds is presented here. These sensors consist of thin polymer foils with laccase-clad etched nuclear tracks. The presence of suitable phenolic compounds in the sensors leads to the formation of enzymatic reaction products in the tracks, which differ in their electrical conductivities from their precursor materials. These differences correlate with the concentrations of the phenolic compounds. Corresponding calibration curves have been established for a number of compounds. The sensors thus produced are capable to cover between 5 and 9 orders of magnitude in concentration in the best case down to some picomoles. The sensor's detection sensitivity strongly depends on the specific compound. It is highest for caffeic acid and acid blue 74, followed by ABTS and ferulic acid.
Trvalý link: http://hdl.handle.net/11104/0237280