A miniaturized device for bioluminescence analysis of caspase-3/7 5 activity in a single apoptotic cell

0428917 - ÚIACH 2015 RIV DE eng J - Článek v odborném periodiku
Adamová, Eva - Lišková, Marcela - Matalová, E. - Klepárník, Karel
A miniaturized device for bioluminescence analysis of caspase-3/7 5 activity in a single apoptotic cell.
Analytical and Bioanalytical Chemistry. Roč. 406, č. 22 (2014), s. 5389-5394. ISSN 1618-2642. E-ISSN 1618-2650
Grant CEP: GA ČR(CZ) GA14-28254S
Institucionální podpora: RVO:68081715
Klíčová slova: apoptosis * bioluminescence * single-cell analysis
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 3.436, rok: 2014

Caspases are key enzymes activated during the 14 apoptotic machinery. Apoptosis as a way of programmed cell 15 death becomes deregulated in some pathologies including 16 cancer transformations, neurodegenerative, or autoimmune 17 diseases. Most of the methods available for the detection of 18 apoptosis and caspases provide qualitative information only or 19 quantification data as an average from cell populations or cell 20 lysates. Several reports point to the importance of more accu- 21 rate single-cell analyses in biomedical studies due to hetero- 22 geneity at tissue as well as cell level.
Trvalý link: http://hdl.handle.net/11104/0234138