Measurement of DNA base and nucleotide excision repair activities in mammalian cells and tissues using the comet assay - A methodological overview

0398229 - ÚEM 2014 NL eng J - Článek v odborném periodiku
Azqueta, A. - Langie, S. A. S. - Slyšková, Jana - Collins, A. R.
Measurement of DNA base and nucleotide excision repair activities in mammalian cells and tissues using the comet assay - A methodological overview.
Dna Repair. Roč. 12, č. 11 (2013), s. 1007-1010. ISSN 1568-7864. E-ISSN 1568-7856
Grant ostatní: EU FP6(XE) LSHB-CT-2006-037575
Institucionální podpora: RVO:68378041
Klíčová slova: comet assay * base excision repair * nucleotide excision repair
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.362, rok: 2013

The article is a comprehensive summary of protocols for the comet-based BER- and NER-specific in vitro DNA repair assays that can be applied to a wide spectrum of biological material - cultured cell lines, blood cells, animal tissue samples and human biopsies. Our intention was to provide a detailed and user-friendly account of the assays, including practical tips and recommendations to help in setting them up in order to facilitate comparison of results obtained in different laboratories.
Trvalý link: http://hdl.handle.net/11104/0225746