Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells

0389610 - ÚOCHB 2013 RIV US eng J - Článek v odborném periodiku
Rubert, M. - Ramis, J. M. - Vondrášek, Jiří - Gaya, A. - Lyngstadaas, S. P. - Monjo, M.
Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells.
Journal of Biomaterials and Tissue Engineering. Roč. 1, č. 2 (2011), s. 198-209. ISSN 2157-9083. E-ISSN 2157-9091
Grant ostatní: GA ČR(CZ) GAP302/10/0427
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: proline-rich regions * synthetic peptides * bone formation * mineralization * In Vitro
Kód oboru RIV: EI - Biotechnologie a bionika

Regulation of biomineralization processes are mediated by extracellular matrix proteins that often exhibit proline-rich regions. Advanced bioinformatic methods were used to design the structure of artificial peptides (P1, P2, P3) based on proline-rich domains of extracellular proteins. Their effect on osteoblast differentiation and in vitro biomineralization was tested on MC3T3-E1 and human umbilical cord mesenchymal stem cells (hUCMSCs) and compared to the commercially available enamel matrix derivative (EMD). MC3T3-E1 and hUCMSCs treated with the synthetic peptides showed a decreased cytotoxicity after 24-48 h of treatment compared to control. MC373-E1 cells treated with EMD showed lower expression of osteoblast markers genes than cells treated with P2, except for collagen type I. In hUCMSCs, OC gene expression was higher in P2-treated cells compared to those treated with EMD or control. ALP activity was markedly increased in MC3T3-E1 cells incubated with P2 compared to other treatments. Similar results were observed in hUCMSCs. Further, P2 increased calcium deposition rate compared to EMD or control either in MC3T3-E1 or hUCMSCs. The observed effects of proline-rich peptides hold potential for both clinical applications and as a research tool in further investigations of the molecular basis of induced osteogenic cell differentiation.
Trvalý link: http://hdl.handle.net/11104/0218477