Efficient biolistic transformation of the moss Physcomitrella patens

0370792 - ÚEB 2012 RIV NL eng J - Článek v odborném periodiku
Šmídková, Markéta - Holá, M. - Angelis, Karel
Efficient biolistic transformation of the moss Physcomitrella patens.
Biologia Plantarum. Roč. 54, č. 4 (2010), s. 777-780. ISSN 0006-3134. E-ISSN 1573-8264
Grant CEP: GA ČR GA521/04/0971; GA AV ČR IBS5038304; GA MŠMT 1M0505; GA MŠMT(CZ) LC06004
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Helios biolistic gun * moss protonemal tissue * particle size
Kód oboru RIV: EI - Biotechnologie a bionika
Impakt faktor: 1.582, rok: 2010

High rates of homologous recombination (HR) in comparison to other plants make the moss Physcomitrella patens an attractive model organism for genetic studies as well as biotechnological applications. We describe a simple protocol for the efficient biolistic transformation of protonemal tissue with minimum tissue handling steps. The transformation efficiency depends on the biolistic conditions. The bombardment of tissue with 1 mu m gold particles yielded between 20 and 40 stable transformants per 1 mu g of DNA. Transformation with circular plasmids generates higher frequencies of random transgene integration, whereas linear plasmids are more efficient in generating gene-targeted insertions.
Trvalý link: http://hdl.handle.net/11104/0204485