Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy

0369489 - FGÚ 2012 RIV US eng J - Článek v odborném periodiku
Michálek, Jan - Čapek, Martin - Kubínová, Lucie
Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy.
Microscopy Research and Technique. Roč. 74, č. 9 (2011), s. 831-838. ISSN 1059-910X. E-ISSN 1097-0029
Grant CEP: GA ČR(CZ) GA102/08/0691; GA ČR(CZ) GA304/09/0733; GA MŠMT(CZ) LC06063; GA MŠMT(CZ) ME09010
Výzkumný záměr: CEZ:AV0Z50110509
Klíčová slova: confocal laser scanning microscopy * image enhancement * morphology filters
Kód oboru RIV: JC - Počítačový hardware a software
Impakt faktor: 1.792, rok: 2011

In images acquired by confocal laser scanning microscopy (CLSM), regions corresponding to the same concentration of fluorophores in the specimen should be mapped to the same grayscale levels. However, in practice, due to multiple distortion effects, CLSM images of even homogeneous specimen regions suffer from irregular brightness variations, e.g., darkening of image edges and lightening of the center. We present a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain
Trvalý link: http://hdl.handle.net/11104/0203541