Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production

0364101 - ÚEB 2012 RIV DE eng J - Článek v odborném periodiku
Plchová, Helena - Moravec, Tomáš - Dědič, P. - Čeřovská, Noemi
Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production.
Journal of Phytopathology - Phytopathologische Zeitschrift. Roč. 159, č. 2 (2011), s. 130-132. ISSN 0931-1785. E-ISSN 1439-0434
Grant CEP: GA MŠMT 1M06030; GA MZe QH71123
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Potato leafroll virus * recombinant viral antigen * antibody production
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 0.791, rok: 2011

Vector pMPM-A4 Omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp(43-616) and RdRp(304-537) were chosen for expression. A high level of CP and RdRp(304-537) was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.
Trvalý link: http://hdl.handle.net/11104/0199670