MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process

0359407 - BC 2012 RIV US eng J - Článek v odborném periodiku
Ammerman, M. L. - Hashimi, Hassan - Novotná, Lucie - Číčová, Zdeňka - Mcevoy, S. M. - Lukeš, Julius - Read, L. K.
MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process.
RNA. Roč. 17, č. 5 (2011), 865-877. ISSN 1355-8382. E-ISSN 1469-9001
Grant CEP: GA ČR GA204/09/1667; GA MŠMT 2B06129; GA MŠMT LC07032
Výzkumný záměr: CEZ:AV0Z60220518
Klíčová slova: RNA editing * trypanosome * MRB1 complex * mitochondria * kinetoplast
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.095, rok: 2011

In this study we examined the function of the mitochondrial RNA-binding complex 1 (MRB1) protein of Trypanosoma brucei, Tb927.5.3010, which we term MRB3010. We show that MRB3010 is essential for growth of both procyclic form and bloodstream form life-cycle stages of T. brucei. Down-regulation of MRB3010 by RNAi leads to a dramatic inhibition of RNA editing, yet its depletion does not impact total gRNA levels. Rather, it appears to affect the editing process at an early stage, as indicated by the accumulation of pre-edited and small partially edited RNAs. MRB3010 is present in large (>20S) complexes and exhibits both RNA-dependent and RNA-independent interactions with other MRB1 complex proteins. Comparison of proteins isolated with MRB3010 tagged at its endogenous locus to those reported from other MRB1 complex purifications strongly suggests the presence of an MRB1 "core'' complex containing five to six proteins, including MRB3010.
Trvalý link: http://hdl.handle.net/11104/0197204