Loss of the response regulator CtrA causes pleiotropic effects on gene expression but does not affect growth phase regulation in Rhodobacter capsulatus

0356033 - ÚMG 2011 RIV US eng J - Článek v odborném periodiku
Mercer, R.G. - Callister, S.J. - Lipton, M.S. - Pasa-Tolic, L. - Strnad, Hynek - Pačes, Václav - Beatty, J.T. - Lang, A.S.
Loss of the response regulator CtrA causes pleiotropic effects on gene expression but does not affect growth phase regulation in Rhodobacter capsulatus.
Journal of Bacteriology. Roč. 192, č. 11 (2010), s. 2701-2710. ISSN 0021-9193. E-ISSN 1098-5530
Grant CEP: GA MŠMT(CZ) 1M0520
Výzkumný záměr: CEZ:AV0Z50520514
Klíčová slova: Gene transfer agent * ctrA regulator protein * gene expression
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.726, rok: 2010

The CtrA protein in Caulobacter crescentus has been thoroughly studied for its role as an essential, master regulator of the cell cycle. Although the CtrA protein in R. capsulatus shares a high degree of sequence similarity with the C. crescentus protein, it is nonessential and clearly plays a different role in this bacterium. We have used transcriptomic and proteomic analyses of wild-type and ctrA mutant cultures to identify the genes dysregulated by the loss of CtrA in R. capsulatus. We have also characterized gene expression differences between the logarithmic and stationary phases of growth. Loss of CtrA has pleiotropic effects, with dysregulation of expression of approximately 6% of genes in the R. capsulatus genome.
Trvalý link: http://hdl.handle.net/11104/0194661