Fluorescent in situ hybridization of mitochondrial DNA and RNA

0355103 - FGÚ 2011 RIV PL eng J - Článek v odborném periodiku
Alán, Lukáš - Zelenka, Jaroslav - Ježek, Jan - Dlasková, Andrea - Ježek, Petr
Fluorescent in situ hybridization of mitochondrial DNA and RNA.
Acta Biochimica Polonica. Roč. 57, č. 4 (2010), s. 403-408. ISSN 0001-527X. E-ISSN 1734-154X
Grant CEP: GA ČR GAP302/10/0346; GA ČR GPP304/10/P204; GA AV ČR KJB500110902
Výzkumný záměr: CEZ:AV0Z50110509
Klíčová slova: mitochondrial DNA and RNA * nucleoids of mitochondrial DNA * molecular beacon fluorescent hybridization probes
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.234, rok: 2010

To reveal nucleic acid localization in mitochondria, we designed molecular beacon fluorescent probes against: i) the light strand complementary to ND5 mitochondrial DNA (mtDNA) gene (annealing also to corresponding mRNA); ii) displacement (D) loop 7S DNA (annealing also to parallel heavy strand mtDNA and corresponding light strand transcript); iii) the proximal D-loop heavy strand displaced by the light strand promoter minor RNA. Confocal microscopy demonstrated ND5 probe spreading (less for other probes) in mitochondrial reticulum tubules but upon RNase A treatment all probes contoured mtDNA nucleoid localization. DNase I spread the signal over mitochondrial tubules
Trvalý link: http://hdl.handle.net/11104/0193946