Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry

0354289 - MBÚ 2011 RIV US eng J - Článek v odborném periodiku
Vidová, V. - Pól, Jaroslav - Volný, Michael - Novák, Petr - Wiedmer, S. K. - Holopainen, J.
Vizualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry.
Journal of Lipid Research. Roč. 51, č. 8 (2010), s. 2295-2302. ISSN 0022-2275. E-ISSN 1539-7262
Grant CEP: GA MŠMT LC07017
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: ocular lens * sphingolipid * matrix-assisted laser desorption/ionization
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 6.115, rok: 2010

The intraocular lens contains high levels of both cholesterol and sphingolipids, which are believed to be functionally important for normal lens physiology. The aim of this study was to explore the spatial distribution of sphingolipids in the ocular lens using mass spectrometry imaging (MSI). Matrix-assisted laser desorption/ionization (MALDI) imaging with ultra high resolution Fourier transform ion cyklotron resonance mass spectrometry (FT-ICR MS) was used to visualize the lipid spatial distribution. Equatorially-cryosectioned, 12 um thick slices of tissue were thaw-mounted to an indium-tin oxide (ITO) glass slide by soft-landing to an ethanol layer. After the automated MALDI matrix deposition, the entire lens section was examined by MALDI MSI in a 150 um raster. We obtained spatial and concentration-dependent distributions of seven lens sphingomyelins (SM) and two ceramide-1-phosphates (CerP), which are important lipid second messengers
Trvalý link: http://hdl.handle.net/11104/0193327