Separation of PCBs by liquid chromatography on reversed phase sub-2-micron particle columns

0354246 - MBÚ 2011 RIV NL eng J - Článek v odborném periodiku
Olšovská, Jana - Křesinová, Zdena - Flieger, Miroslav - Cajthaml, Tomáš
Separation of PCBs by liquid chromatography on reversed phase sub-2-micron particle columns.
Talanta. Roč. 80, č. 5 (2010), s. 1849-1855. ISSN 0039-9140. E-ISSN 1873-3573
Grant CEP: GA MŠMT 2B06156; GA ČR GA525/09/1058
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Ultra high-performance liquid chromatography * PCB * Congener analysis
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.722, rok: 2010

A new ultra high-performance liquid chromatography method with UV detection was examined for detection and separation of polychlorinated biphenyls. This included optimization of separation conditions for two model mixtures containing seven and fifteen most relevant congeners, comparison of three types of reversed phase sub-2-micron particle sized columns and assessment of system suitability under the optimized conditions. Calibration curves determined in the range from 0.5 to 50.0_g/mL exhibited correlation coefficients ranging from 0.997 to 0.999. Lower limits of detection ranged from 0.1 to 0.5 ppm. The most efficient Grace C18 column filled with 1.5_m particles was then tested to separate the complex commercial mixture Delor 103, where the elution order was confirmed by GC–MS. 13 individual congeners were separated and some of the other co-eluting congeners could be resolved using another separation dimension performed with a mass spectrometry detector
Trvalý link: http://hdl.handle.net/11104/0193294