Newly designed, simple relief phase contrast for microscopy of microorganisms

0352834 - MBÚ 2011 RIV CZ eng J - Článek v odborném periodiku
Žižka, Zdeněk
Newly designed, simple relief phase contrast for microscopy of microorganisms.
Folia Microbiologica. Roč. 55, č. 6 (2010), s. 662-665. ISSN 0015-5632. E-ISSN 1874-9356
Grant CEP: GA MŠMT LC06066
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: optical microscopy * Zeiss NG 10/1 * microphotography
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 0.977, rok: 2010

A new method providing a relief phase contrast for investigation of microorganisms by optical microscopy used a neutral filter Zeiss NG 10/1 that could be controllably slid at a certain azimuthal angle below the aperture condenser diaphragm of the microscope phase contrast. Two ways of application are described depending on the type of the microscope: (1) in a special holder, and (2) fixed on a rubber ring. The device enabled us to obtain excellent results in the area of both optical microscopy and microphotography. With the microorganisms vizualized, a better resolution, higher contrast and a significant 3D effect were obtained; outer morphology and organelles (chloroplasts, nuclei, granules, oil reserve vacuoles, etc.) could also be investigated
Trvalý link: http://hdl.handle.net/11104/0192245