Biotransformations with nitrilases

0352304 - MBÚ 2011 RIV GB eng J - Článek v odborném periodiku
Martínková, Ludmila - Křen, Vladimír
Biotransformations with nitrilases.
Current Opinion in Chemical Biology. Roč. 14, č. 2 (2010), s. 130-137. ISSN 1367-5931. E-ISSN 1879-0402
Grant CEP: GA AV ČR IAA500200708; GA MŠMT(CZ) LC06010; GA MŠMT OC09046; GA MPO FT-TA5/043
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: ENZYME-PROMOTED DESYMMETRISATION * PSEUDOMONAS-FLUORESCENS EBC191 * SUBSTRATE-SPECIFICITY
Kód oboru RIV: CE - Biochemie
Impakt faktor: 9.312, rok: 2010

The range of possible nitrilase applications has been recently broadened but in most cases the parameters of the reactions need to be improved to establish viable industrial processes. To achieve this goal, several methods have been used, primarily in screening for enzymes from new sources, enzyme improvement, substrate structure modification, medium engineering, and variation in process parameters. New nitrilases have been obtained by for example genome mining and the metagenomic approach. Protein engineering revealed targets for altering substrate specificity, activity or enantioselectivity, and for changing the acid:amide ratio in the nitrilase product
Trvalý link: http://hdl.handle.net/11104/0191839