Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes

0342763 - ÚFCH JH 2011 RIV US eng J - Článek v odborném periodiku
Macháň, Radek - Hof, Martin
Recent Developments in Fluorescence Correlation Spectroscopy for Diffusion Measurements in Planar Lipid Membranes.
International Journal of Molecular Sciences. Roč. 11, č. 2 (2010), s. 427-457. E-ISSN 1422-0067
Grant CEP: GA ČR GA203/08/0114; GA AV ČR GEMEM/09/E006
Výzkumný záměr: CEZ:AV0Z40400503
Klíčová slova: lateral diffusion * fluorescence fluctuation spectroscopy * confocal microscopy
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 2.279, rok: 2010

Fluorescence correlation spectroscopy (FCS) is a single molecule technique used mainly for determination of mobility and local concentration of molecules. This review describes the specific problems of FCS in planar systems and reviews the state of the art experimental approaches such as 2-focus, Z-scan or scanning FCS, which overcome most of the artefacts and limitations of standard FCS. We focus on diffusion measurements of lipids and proteins in planar lipid membranes and review the contributions of FCS to elucidating membrane dynamics and the factors influencing it, such as membrane composition, ionic strength, presence of membrane proteins or frictional coupling with solid support.
Trvalý link: http://hdl.handle.net/11104/0185401