Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.)

0340099 - BC 2010 RIV US eng J - Článek v odborném periodiku
Matoušek, Jaroslav - Kocábek, Tomáš - Patzak, J. - Stehlík, Jan - Füssy, Zoltán - Krofta, K. - Heyerick, A. - Roldán-Ruiz, I. - Maloukh, L. - De Keukeleire, D.
Cloning and Molecular Analysis of HlbZip1 and HlbZip2 Transcription Factors Putatively Involved in the Regulation of the Lupulin Metabolome in Hop (Humulus lupulus L.).
Journal of Agricultural and Food Chemistry. Roč. 58, č. 2 (2010), s. 902-912. ISSN 0021-8561. E-ISSN 1520-5118
Grant CEP: GA ČR GA521/08/0740; GA MZe QH81052; GA MŠMT ME 940
Výzkumný záměr: CEZ:AV0Z50510513
Klíčová slova: secondary metabolites transcriptional regulation * cDNA-AFLP analysis * hop cDNA library screening * Nicotiana benthamiana * Petunia hybrida
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.816, rok: 2010

TFs HlbZIP1A and HlbZIP2 cloned from a cDNA library based on glandular tissue-enriched hop cones are similar to the group A3 bZIP TFs according to characteristic domains. While HlbZIP1A is rather neutral (pl 6.42), HlbZIP2 is strongly basic (pl 8,51). Both bZIP TFs show specific expression in lupulin glands, although low expression was observed also in roots and immature pollen. In a transient expression system, both hop bZIP TFs activated the GUS-coupled promoters of chalcone synthase chs_H1 and O-methyl transferase 1, while the response of the valerophenone synthase promoter to the cloned hop TFs was very low, showing that the cloned bZIP TFs are not strictly G-box-specific. HPLC analysis of secondary metabolites in infiltrated Petunia hybrida showed that both bZIPs interfere with the accumulation and the composition of flavonol glycosides, phenolic acids, and anthocyanins, suggesting the possibility of coregulating flavonoid biosynthetic pathways in hop glandular tissue.
Trvalý link: http://hdl.handle.net/11104/0183418