Extraction and separation of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species by CZE

0336576 - ÚOCHB 2010 RIV DE eng J - Článek v odborném periodiku
Sázelová, Petra - Kašička, Václav - Ibanez, E. - Cifuentes, A.
Extraction and separation of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species by CZE.
Journal of Separation Science. Roč. 32, č. 21 (2009), s. 3801-3808. ISSN 1615-9306. E-ISSN 1615-9314
Grant CEP: GA ČR(CZ) GA203/08/1428
Grant ostatní: GA ČR(CZ) GA203/09/0675
Program: GA
Výzkumný záměr: CEZ:AV0Z40550506
Klíčová slova: Bacillus thuringiensis-transgenic maize * CZE-UV profiling * Maize proteins
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 2.551, rok: 2009

A new method for extraction and capillary electrophoretic (CE) separation and characterization of water-soluble proteins (albumin fraction) from Bacillus thuringiensis-transgenic and non-transgenic maize varieties was developed. The best CE separation of extracted proteins was achieved in isoelectric background electrolyte (200mM iminodiacetic acid, pH 2.26). Well resolved components of protein extracts were characterized by effective electrophoretic mobilities and corrected peak areas. Significant qualitative and quantitative differences in CE-UV profiling were found, which could potentially be used to distinguish the B. thuringiensis variety from the wild maize species.
Trvalý link: http://hdl.handle.net/11104/0180780