Insects Pathogens: Molecular Approaches and Techniques

0335280 - BC 2010 RIV GB eng M - Část monografie knihy
Oborník, Miroslav
Identification and Diagnostics of Entomopathogenic Protozoa.
Insects Pathogens: Molecular Approaches and Techniques. Wallingford: CAB International, 2009 - (Stock, S.; Vandenberg, J.; Glazer, I.; Boemare, N.), s. 101-128. ISBN 978-1-84593-478-1
Výzkumný záměr: CEZ:AV0Z60220518
Klíčová slova: entomopathogenic protozoa * diagnostics * strains specific PCR
Kód oboru RIV: EB - Genetika a molekulární biologie

Molecular methods allow us to recognize and identify target organisms from a variety of environments including laboratory cultures, host tissues or environmental samples. Ascending progress on the field of molecular evolution during last 15 years greatly influenced high order taxonomy. It has been shown that the proposed kingdom Protozoa comprising unicellular heterotrophic eukaryotes is polyphyletic in its nature and its taxonomic relevance is no longer sustainable. Here I show the two up-to-date proposals dividing eukaryotic organisms into five and six major groups (kingdoms) respectively. These are currently the most accepted views on protist and eukaryotic high order taxonomy referred by Simpson and Roger (2004) and Keeling et al., (2005). This chapter follows methods for molecular identification of Protozoa colonizing insects and their molecular diagnostics.
Trvalý link: http://hdl.handle.net/11104/0179787